FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30034534
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT7683152.5581052797423127TruSeq Adapter, Index 20 (97% over 44bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1879580.6258062801973222Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1097380.3653727405925459Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1069590.3561200583301875No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG799740.26627348371711046Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT644280.21451306685830387No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC606120.20180769243831118Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT583900.19440954202918548No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT519970.17312404447493676No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC500280.16656825772625605No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC474880.1581113261154643No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC400620.13338645440611796No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA389490.1296807202002868No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT380800.12678738414919308TruSeq Adapter, Index 1 (97% over 35bp)
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC355880.11849026856884146No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT353250.11761460990205475No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA346530.11537718547589251No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT332200.11060601106712693Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA302590.1007473596893496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)