FastQCFastQC Report
Wed 27 Sep 2023
EGAF00008245182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120424510
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT11253850.9345149089666214TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA3403980.2826650488343278TruSeq Adapter, Index 27 (97% over 44bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2374310.19716169075547824No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2331700.19362337451072045No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1875600.15574902484552355No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1612540.13390463452996404No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1391400.11554126315315712No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1358210.11278517969473159No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)