FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26454194
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC11129084.206924618455584TruSeq Adapter, Index 5 (100% over 50bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1442430.5452556974519805No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1369650.5177439917466395No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA578750.21877438412979053No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT554550.209626496275033No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC516870.1953830080780386Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT470350.1777978947307939No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT463700.1752841156302097No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG449570.1699428075563368No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC440210.16640461622077768No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC432430.1634636836790416No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC414360.1566330087395594No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT414180.15656496659849098No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA412420.1558996656635995No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG370530.14006474738939315No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC362640.13708223353922633No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT349960.13228904271284925No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA329870.12469478374582117No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG324510.12266863998956082Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC302490.1143448180655211Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA296650.11213722859974491No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC282180.1066673964816316No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA280110.1058849118593445No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG279810.10577150829089708No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA274510.10376804524832622No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG270190.10213503386268354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)