FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40294302
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC18511904.594173141403466TruSeq Adapter, Index 5 (100% over 50bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2215700.5498792360269698No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC2018080.5008350808508856No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC945400.2346237440718045Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA868700.2155887946638212No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT817820.20296169915041584No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT763410.18945854925095862No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG712480.17681904503520127No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT684390.16984783605384204No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC656860.16301560453882538No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC643140.15961065661343382No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC632160.15688570557693243No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT623760.15480104358179478No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA619920.15384805524116038No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG618680.15354031942283056Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG592420.14702326894755494No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT565580.14036227752499597No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC538320.13359705300268013No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC503910.12505738404402686Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA494440.12270717581855618No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC463880.11512297694100768Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG457060.11343042994019353No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA449750.11161627765632967No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA447580.11107773997425244No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA446970.11092635380555792No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG424920.10545411606832152No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC424310.105302729899627No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC408580.10139895213968465TruSeq Adapter, Index 5 (98% over 50bp)
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG407390.10110362502370683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)