FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22290989
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC4664032.0923387472848334TruSeq Adapter, Index 1 (100% over 50bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1103460.49502514222226746No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC690690.30985166248119367Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC671580.3012786915825045Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC602070.2700956875444154No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT471620.21157428232547243No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT467100.20954655713122466No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC418240.18762738611552857No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT360460.1617065981235736TruSeq Adapter, Index 1 (100% over 35bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA344710.15464096276751113No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT342600.15369439193568307No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG337130.15124048556122835TruSeq Adapter, Index 1 (100% over 39bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT306960.1377058685013931No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT300720.13490653106508643No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG291530.13078378891129505No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA280460.12581765663246255TruSeq Adapter, Index 1 (100% over 37bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG266520.11956400857763645No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA264930.11885071586550064No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC253350.1136557915846623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)