FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77684706
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC47149066.069284731540337Illumina Single End PCR Primer 1 (100% over 50bp)
AGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT1117510.1438519957840865No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT975510.1255729795772156No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT941360.12117700490492941No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC843590.10859151607010006No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)