FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8534714
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG118441113.877571058620125Illumina Single End PCR Primer 1 (100% over 50bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG734160.8602045715884563Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG420980.4932561301995591Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA413600.4846090917633561Illumina Single End PCR Primer 1 (100% over 34bp)
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT351400.41173025833085913No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG317040.37147114712924184Illumina Single End PCR Primer 1 (100% over 28bp)
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG304450.35671962762899845No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA208440.24422610997861205No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA168510.19744071095996887Illumina Single End PCR Primer 1 (100% over 26bp)
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT166870.19551914686303493No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT152480.17865859359786396No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA149330.17496778450924072No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC141710.16603954157104736No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC140180.16424686287085893No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC131390.15394774798546265No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA130610.15303383335399404Illumina Single End PCR Primer 1 (100% over 36bp)
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT124590.14598028709573632No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG123230.14438679491779102No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT123190.14433992750079266No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT122640.14369550051706478No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT122470.14349631399482163No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG113880.1334315361944173Illumina Single End PCR Primer 1 (100% over 24bp)
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG113280.13272852493944143No Hit
CTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCT110420.12937750462405653No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC106470.12474934719546547No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC106460.12473763034121588No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG102960.12063673135385673No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG93880.10999782769522212No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT93060.10903704564675512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)