FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84160533
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA41448664.924952174435492TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT13317941.5824448260088848TruSeq Adapter, Index 16 (97% over 40bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2861540.3400097287881958No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2305340.2739217442931356No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC2035040.24180455225966785No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTA1956920.2325222916542128TruSeq Adapter, Index 16 (97% over 40bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1924360.22865349486320385No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1251570.14871222357871713No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1133140.13464030699520405No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1096600.1302986044539428No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1085010.12892147439227838No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG979980.11644175304830828No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTA923760.10976166227464362TruSeq Adapter, Index 16 (97% over 44bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT916310.10887644924967384No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)