FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13655639
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG481040.3522647310755652Illumina Single End PCR Primer 1 (100% over 32bp)
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAG356870.26133526230445897No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG323090.23659822876102685Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA285040.20873428185967718Illumina Single End PCR Primer 1 (100% over 34bp)
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG243010.17795578808139262No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG241490.17684269480175918Illumina Single End PCR Primer 1 (100% over 28bp)
CCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGC226480.1658508986653792No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG177630.13007813109294997No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT173970.12739791964330632No Hit
GGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCG167030.12231577006392744No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT158860.11633289368589782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)