FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43591453
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT7313681.677778439732211TruSeq Adapter, Index 15 (97% over 40bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC3943580.9046681696983121No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2477520.5683499469494628No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1921520.44080200767797306Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1810390.41530847801746823No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1771770.40644894309900614No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1593870.3656381905874989No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1565430.3591139758521011No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1516250.3478319476985546No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1356110.31109538835514383No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1317570.30225420565815964No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1272840.29199301982432196No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1105270.25355199791115013No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1099620.2522558722692726No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1096880.2516273086836541Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG891800.2045813889250262Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC855870.19633894745375888No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG815690.1871215442164775No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG806980.1851234461030698No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT715790.16420420764593463No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC707570.16231851688907914Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG641650.14719628639127952No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT616840.14150480370544197No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT441340.10124461783827211TruSeq Adapter, Index 1 (100% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT437450.10035224106890862Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)