FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60561757
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT8417631.389924998378102TruSeq Adapter, Index 27 (97% over 44bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT4697370.7756330451244999No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC4193790.692481560599373No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2980590.49215712153133206No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2166430.35772244850822277No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2139480.3532724455137588Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1975860.3262553957937515No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1734320.28637214075542755No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1681010.27756955598233385No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1637930.27045615601938366No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1636560.27022994065380235No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1588560.2623041468232172No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1498680.2474630978754464Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1495650.2469627821398907No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1188170.19619146782680033No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA989890.16345133447829133No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG945390.1561034631145196No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG907980.14992629754780729Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC882010.14563811284405107No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG791080.13062368715623623No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT774870.1279470805313657TruSeq Adapter, Index 1 (100% over 35bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG702970.11607490185596829No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA679130.11213842425344431No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC635770.104978790493149Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAA623060.10288010633509195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)