FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42569493
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT12051902.8311119420661175TruSeq Adapter, Index 14 (97% over 44bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2863630.6726953501654342Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2101860.49374795231881197Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1457010.3422662327690865Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC861260.20231859467999772Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG778890.18296905720723522TruSeq Adapter, Index 2 (97% over 35bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC534700.12560638201634208No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT531370.12482413168510136No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCC518250.12174211236201474TruSeq Adapter, Index 14 (100% over 39bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT515180.12102093863321324TruSeq Adapter, Index 8 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)