FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42569493
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG12337672.8982421754470975Illumina Single End PCR Primer 1 (100% over 50bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2914710.6846945534446464Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2140980.5029376318858202Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1461410.3432998368103656Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG826370.19412258445267366Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA790560.1857104570167185Illumina Single End PCR Primer 1 (100% over 36bp)
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG598070.14049262931085413No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC522790.12280860380460722Illumina Single End PCR Primer 1 (100% over 40bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC519640.12206863727505517Illumina Single End PCR Primer 1 (100% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)