FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87064346
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA35223874.045728431704982TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT9534631.0951245185945577TruSeq Adapter, Index 25 (97% over 44bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA6713880.7711400025907276No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT6338440.7280178731256995No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC6167920.7084323587522268No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT4339420.49841527552506976No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC3163570.36335999124142043No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2966210.3406916994472111No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2649030.3042611725355406No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2212570.2541304335990762No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG1768160.2030865769094504No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA1502900.17261945550018834No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT1253920.1440222154772747No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT1187750.13642208947391624No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1126460.12938246845614623No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1100710.1264248857965349No Hit
CTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTC1041120.11958052266308876No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1003750.11528829493533438No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG995500.11434071990846861No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC950220.10913996873071324No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG932270.10707827518741139No Hit
CGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG919090.10556445229600644No Hit
GTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGC916480.10526467401477983No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT912020.10475240921237723No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)