FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74675483
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT8362641.1198641996061813TruSeq Adapter, Index 25 (97% over 44bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2389480.31998186071324103No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1764090.23623416001206177No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1622080.21721720902695735Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1286460.17227340866345653No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1013410.13570852966561997Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT971870.13014579363350084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)