FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74675483
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG8627901.1553858982070462Illumina Single End PCR Primer 1 (100% over 50bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1656020.2217622080864211Illumina Single End PCR Primer 1 (100% over 32bp)
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT1063570.14242559368514562No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1041680.13949424337837893Illumina Single End PCR Primer 1 (100% over 34bp)
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC1031960.13819261135545652No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA765940.10256913905732622No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT750430.10049215215655184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)