FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33555671
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT22870926.815813636985533TruSeq Adapter, Index 13 (97% over 40bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC7550822.250236629152789Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6149891.8327423701346934Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG4118511.2273663071735326Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG2941520.8766089046468479TruSeq Adapter, Index 2 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC2714440.8089362897854135Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTC1909870.569164598139015TruSeq Adapter, Index 13 (100% over 37bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAA1618010.48218675168200337TruSeq Adapter, Index 13 (100% over 39bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT1429740.42607999106916983Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT900400.2683302026652961No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC886010.26404180682305534No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA750350.2236134690914093Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACA687840.20498472523467048TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGC669120.19940593648090066No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT667850.19902746096181478No Hit
GGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGA666810.19871752825327202No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT649260.1934874137966128No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA573640.17095172973891656No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA569720.16978352183748613No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG470970.14035481513691084No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA419950.12515023168513006No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC408330.12168732969160415No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC396750.11823634818686833No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT382690.1140462963771459No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG364350.1085807522668821No Hit
GGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCG340440.10145528009259598No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)