FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37989403
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT21769215.73033748385043TruSeq Adapter, Index 15 (97% over 40bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC4464321.1751487645120402Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2952410.7771667272581251Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG2589310.6815874416347105Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1999690.5263810015650944No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1742990.4588095264355694No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC1726860.4545636055402082Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1226710.32290847002781276TruSeq Adapter, Index 1 (100% over 35bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1185000.31192909243664607No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1179940.31059714205037653No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1015110.2672087266019948No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGT982370.2585905337864878TruSeq Adapter, Index 15 (100% over 37bp)
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT855750.22526018637355266No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCA855370.22516015847893162TruSeq Adapter, Index 15 (100% over 39bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT849840.22370448938089393No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG805010.21190383012862826No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT775510.20413850673041636Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT756420.1991134211822176No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA699750.18419610331860178No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG676830.1781628418851436No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC623320.16407733493469218No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC600270.15800985343202156No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC598030.15742021531636072No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC582610.15336118864515982No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA576830.15183971172171357No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC547120.1440191097501585No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA541950.14265820392070916No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG433210.11403443218099532No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG400610.10545309174771712No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)