FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43130270
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT21484994.981417922957589TruSeq Adapter, Index 18 (97% over 40bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC3407270.7899950545173957No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2727010.6322728793490048Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1889220.43802647189549243No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1775160.4115810079556655Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1547180.3587225398774457No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1492930.34614436682172406Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1254440.29084909507869994No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1236360.28665714357920785No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1086800.25198080141858603No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC1021130.2367548359887383Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC983320.22798837104428052No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC892600.2069544197149705No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT749610.1738013696645071TruSeq Adapter, Index 1 (97% over 35bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT682320.15819979796092162No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG644890.14952143819178504No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC610530.14155487549695375No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCC558840.12957025309602746TruSeq Adapter, Index 18 (100% over 37bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT518710.12026588287066137No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT493780.11448571965814265No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG492170.11411243194165027No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT479070.11107512195031471Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA472090.10945676899309928No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGC435600.10099635360502034TruSeq Adapter, Index 18 (100% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)