FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49036711
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT6960841.419516084592215TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2055250.4191247655251593Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1984520.40470087808295296Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1851690.37761300915960694No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1562270.31859192187665275No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1166070.23779531216928476No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA1068770.21795303522701595TruSeq Adapter, Index 7 (97% over 35bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT850860.1735148998879635No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT807590.16469089862083125No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA803130.16378137595729045No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA743800.151682277386018No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC733490.14957977095976113No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG681630.13900402088549538Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA664750.13556170192572664No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT648700.13228864391006975TruSeq Adapter, Index 7 (97% over 36bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG626750.12781240568928043No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG615560.12553044187649534TruSeq Adapter, Index 7 (97% over 36bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT607330.12385210745475977No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC578750.1180238209695589No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA498060.10156880219801037No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG496580.10126698750248563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)