FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49036711
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6417621.3087378556037332Illumina Single End PCR Primer 1 (100% over 50bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2206910.45005261466251273Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2127830.43392592133677155Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA1114570.22729297648041688Illumina Single End PCR Primer 1 (100% over 36bp)
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG1091270.2225414343143854No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1035830.21123561896310705No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT982640.20038864352056565No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC870860.17759347685451415No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT859620.17530131659931272No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG746740.15228182820010094Illumina Single End PCR Primer 1 (100% over 30bp)
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT731500.14917395255158936No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA722750.14738957512872344No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT700340.14281952963770347No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA681700.1390182959048783No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC669280.13648549960865033Illumina Single End PCR Primer 1 (100% over 38bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC616050.12563036701217584Illumina Single End PCR Primer 1 (100% over 40bp)
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA615660.1255508347613281No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC597940.12193721556896424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)