FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70741851
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT4372130.618040090582306No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC3499280.49465485430965045No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA3436620.48579729699184715No Hit
CCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCC2961550.41864185883403027No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT2774170.39215400230338904No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC2335240.33010727977700216No Hit
CCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTC2249210.31794616174236096No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT2245240.31738496636170854No Hit
TGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG2173130.30719156613529947No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT2031600.28718502149456054No Hit
AGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGA1774580.250852921561241No Hit
AGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCG1742920.24637749441981666No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1702110.24060863208117075Illumina Single End PCR Primer 1 (100% over 50bp)
GCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGC1439770.20352450206596942No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1397020.19748140319370494No Hit
GTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAG1396610.1974234459881464No Hit
GGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGG1378040.1947984086534575No Hit
AGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGA1359590.19219033440332228No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1353850.19137893352550248No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC1343180.18987063259060044No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCC1328280.1877643829251796No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG1312270.18550122472763683No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA1264810.17879232478663867No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA1206720.1705807782722564No Hit
GCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGT1119580.15826275170549325No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1084010.15323461072569333No Hit
CTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTG1065760.15065480828314767No Hit
GTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAG1000550.14143678541857777No Hit
CCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAA989060.13981257007255862No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG943150.1333227766403794No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA864940.12226708628248927No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA826230.11679507792353357No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT812720.11488531732086005No Hit
GGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGG805420.11385339634384177No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT776810.1098091142681579No Hit
CCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC767510.10849447521524423No Hit
ATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT754680.10668083875837515No Hit
AGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCG744600.10525593965586227No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT741570.10482762177088073No Hit
AGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACTCCCA741330.10479369560177326No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTA734660.10385083081866206No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG727840.10288676217985872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)