FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22104803
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC4349261.9675633390625558TruSeq Adapter, Index 1 (100% over 50bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1228170.5556122802813488No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC963930.4360726490075483No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT935060.42301213903602763No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT818670.3703584239135721No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA729940.33021782641537223No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC729770.33014092005253337Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA663680.30024244052299404No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT662380.2996543330424614No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG607510.27483167346028825No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC605990.27414404009843474No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA589910.2668696029546158No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT584340.26434978859571834No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT544820.24647132118752654TruSeq Adapter, Index 1 (100% over 35bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC491140.22218700614522555Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT456380.20646191689652243No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC445380.20148562283047716No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT406650.18396454381430136No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG390040.17645033977457297No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG383700.1735821848310523No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG339710.1536815324705676TruSeq Adapter, Index 1 (100% over 39bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA312400.14132675147568607TruSeq Adapter, Index 1 (100% over 37bp)
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC304890.13792929979968607No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC293610.13282633643014144No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG288550.13053724115976062No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC265680.12019107340608282No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA263950.1194084380666048No Hit
TGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA256120.11586622147231984No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC246450.11149160659789639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)