FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65011842
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT10727431.6500732281974104TruSeq Adapter, Index 21 (97% over 40bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1358240.20892193763714617No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1291090.19859305017076737No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1254320.19293715751047325Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1219020.18750737750208646No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1195960.18396033141162188No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1184990.18227294651949716No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1165680.1793027184185921Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1138890.17518193070117902No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1037110.1595263213738814No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA971900.14949584108076802No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC968290.1489405576294854No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG780710.12008735270106637No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC736370.11326705679251482No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT688810.10595146650359483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)