FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36725026
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT13431513.6573180370246714TruSeq Adapter, Index 20 (97% over 44bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1667730.45411268054650256Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1159060.3156049501503416No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1153130.3139902474133034Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1116550.3040297371062447No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT968190.2636322163529578No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG885380.2410835597502368Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT880300.23970030681530352No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC783310.21329052292570197No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA700790.1908208315495815No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC593790.1616853858728378Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA590830.16087939597374282No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC583600.15891071118642638No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT579980.15792500732334402No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA492730.13416736587198058No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC449610.12242605355813772No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG427510.1164083587034084No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT418850.11405029366078596TruSeq Adapter, Index 1 (97% over 35bp)
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC410530.11178480853900553No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG400060.10893389156484191No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC396590.10798903178448396No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT386510.1052443094254038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)