FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52952342
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG13187012.490354439847061Illumina Single End PCR Primer 1 (100% over 50bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1563640.295291943838858Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1289100.24344532296607393Illumina Single End PCR Primer 1 (100% over 34bp)
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG840690.15876351606884545No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG728490.13757465156120952Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA663340.12527113531635672Illumina Single End PCR Primer 1 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)