FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26473548
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT17420716.580421332267212TruSeq Adapter, Index 22 (97% over 40bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2896281.0940278953164873Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2647721.0001379490199047Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG1525390.5761940182706149TruSeq Adapter, Index 2 (100% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1471720.5559209517364276Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA1012540.3824723456032414TruSeq Adapter, Index 22 (100% over 37bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC992880.3750460648493356No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC926770.35007396817381636Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT768040.2901160056068042No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACG742940.28063484350492046TruSeq Adapter, Index 22 (100% over 39bp)
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT716480.2706399610660422No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT677990.25610092005801416No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA644870.2435903189100305No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA574020.21682775576586863No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT507030.19152325181347057No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT495570.18719440250320812Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC452960.17109909106252022No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG432710.1634499463388889No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC416790.15743639651171804No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA409120.15453916490528583No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG399460.15089023957045727No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC370680.14001901067435313No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC359230.13569393871950974No Hit
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTA358440.13539552764140267TruSeq Adapter, Index 22 (97% over 40bp)
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC355650.13434164547948013No Hit
GGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGA350590.13243030363742705No Hit
GGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGC324430.12254874186112114No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC321970.12161951242802815No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA315650.11923222380317137No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT283040.10691426778156067No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)