FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26473548
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG18025186.808751135284171Illumina Single End PCR Primer 1 (100% over 50bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2982831.1267209064686003Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2718811.0269911686941244Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA1566730.5918096055730799Illumina Single End PCR Primer 1 (100% over 36bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1446350.5463378010382288Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1029880.3890222798999212Illumina Single End PCR Primer 1 (100% over 38bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG889230.33589377593060066Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC755460.2853640924896051Illumina Single End PCR Primer 1 (100% over 40bp)
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT732690.27676305420036634No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC595040.22476775685676886No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA589910.22282997352678227No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT552010.20851379648847973No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG442180.167027101920755No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA437590.1652932957833986Illumina Single End PCR Primer 1 (100% over 26bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT373930.1412466511855532No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT368310.13912377744003185No Hit
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG367230.1387158230547715Illumina Single End PCR Primer 1 (100% over 42bp)
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG362830.13705378667037754No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT359520.13580348202666298No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC338490.12785970358034365No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC336590.12714200605071901No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT333670.12603901826834846No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC331090.12506446057022655No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC324280.12249208152983498No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA321190.12132487870534013No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG309160.1167807201361903No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT308680.11659940707607458No Hit
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC304790.11513001581805356No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG300700.11358507745165099No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT291350.11005325013481382No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG285270.10775661804001489No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG283580.10711824497419084No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC277710.10490093734319254No Hit
CGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCC274800.10380172691624107No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC270080.10201881515843664No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)