FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26805402
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT17244996.433400998798675TruSeq Adapter, Index 25 (97% over 44bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2893591.0794801734366826Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2441700.9108984823283008Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1545610.5766039248357476Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1475860.5505830503866348TruSeq Adapter, Index 5 (100% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTG1096990.4092421370886361TruSeq Adapter, Index 5 (97% over 37bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC1020320.38063969344686566Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC906820.3382974819776999No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGAT796970.29731693633992134TruSeq Adapter, Index 25 (100% over 39bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT651270.24296222082399657No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT630160.23508694254986365No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA529810.19765045866501088No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT524510.19567324526601018Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA490620.18303027128636237No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC487170.18174321728135245No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC486970.1816686054549751No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT456190.17018584537549558No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA435230.16236652597114565No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT429990.16041169612005818No Hit
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATAT399920.1491938080242184TruSeq Adapter, Index 25 (100% over 41bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC365590.1363866880265403No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC351510.13113401544957243No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA345440.1288695465190188No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC331340.1236094127594132No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG328090.12239697058078071No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC310560.11585724399880294No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA301050.11230945165455829No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG293660.10955254466991392No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC292050.10895191946757599No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)