FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42146304
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT6873421.6308476301978934TruSeq Adapter, Index 18 (97% over 40bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1671060.3964902829913627Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1387640.3292435796979967No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1185120.281191916615037No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC977410.23190882882636638Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT822990.19526979162870367No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG708410.16808354061129535Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC681840.1617793104704982No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA582770.138273097446457No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC537780.12759837730966872Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT522170.1238946124433592No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT488060.11580137608270466No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA446670.105980823371843No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC429560.1019211554113974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)