FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51578809
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT25238124.893118024497231TruSeq Adapter, Index 27 (97% over 44bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3262060.6324419007038337Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2557980.4959362283840249Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC2259200.4380093382923983No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1989940.3858057288604706No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1683900.3264712839724547Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1680990.32590709878547214No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1441880.27954891319805386TruSeq Adapter, Index 1 (100% over 35bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1311310.2542342534508697No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1274160.24703168310846418No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1214130.2353931824986498No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC1082450.20986331809251355TruSeq Adapter, Index 13 (97% over 37bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC1017680.1973058354255524Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA933630.18101038354724322No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT930630.1804287493338592No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC916060.1776039458375241No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC897020.1739125073632468No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT894270.17337934266764476No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC829960.1609110439134025No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCT793150.15377439211518049TruSeq Adapter, Index 27 (100% over 39bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG769380.1491659103644677No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC727370.14102109259638002No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC704240.1365366928111892No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG697550.13523964851534281No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA685860.13297321386385635No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA595680.11548928940953251No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG559490.10847284201540987No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA556360.10786600365277918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)