FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34111253
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT7973802.3375863677596365TruSeq Adapter, Index 7 (97% over 36bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1905090.558493116626352No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1758980.5156597443078388Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1252060.36705189340303623Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1123810.32945432992449736No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC977550.2865769838475297No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC895810.26261421707376154No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT895500.2625233379729557No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT840380.24636444753290068No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG729260.2137886872698578Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA696930.2043108765309794TruSeq Adapter, Index 7 (97% over 35bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC678790.19899298334189014No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA635260.18623179863841413No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC615200.1803510413411082No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA605750.177580694558479No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT600310.1759859129185316No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT534960.1568280121518843TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC523000.15332183781111763Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA504970.148036192044895No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA416080.1219773427847989No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG410470.12033272421860318No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA373190.10940377945072847No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG362220.10618783191576106No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG359200.10530249357887851TruSeq Adapter, Index 7 (97% over 36bp)
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC356410.10448458167162608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)