FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28733984
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT6995332.4345144759598947TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1843980.6417418482588422Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1623410.564979085392405No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1254690.43665716525769627Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT956170.3327662464070419No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT849730.2957230017250653No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC844450.2938854563293416No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG833920.2902208061367334Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC784390.2729833774529839No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT730700.2542981857301793No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA667580.23233116577220897TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC656930.2286247531842434Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC571980.19906045747084705No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA555910.1934677766925742No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC536220.18661526365435435No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT517550.18011773097667208No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA512550.1783776311701155No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT497580.17316777234928507TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT467450.1626819309149751Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA440330.1532436295642122No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA390350.13584959189787257No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA366780.12764676140976483Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG347440.12091605535800395No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA340950.11865740580909352No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG333930.11621430568068807TruSeq Adapter, Index 7 (97% over 36bp)
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC322320.11217379392986368No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG321170.11177357097435565No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG287450.10003833787893807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)