FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28733984
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1880060.6542984084629545Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1281190.44587969423244617Illumina Single End PCR Primer 1 (100% over 34bp)
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT1057720.36810767347820617No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT907590.31585943668653815No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG847000.29477290723068544Illumina Single End PCR Primer 1 (100% over 30bp)
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG768550.26747074126581266No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA682480.23751666319574757Illumina Single End PCR Primer 1 (100% over 36bp)
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG666200.23185089822559934No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG651990.22690553457536553Illumina Single End PCR Primer 1 (100% over 28bp)
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA598310.20822382305217402No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC590100.20536657916980813No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA531700.18504221342922722No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT511960.17817229939294182No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC511250.17792520522041078Illumina Single End PCR Primer 1 (100% over 38bp)
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT488800.1701121570889717No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT465000.16182928200976238No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG451890.15726674031697102No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA451120.1569987649467613Illumina Single End PCR Primer 1 (100% over 26bp)
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT440150.15318098597117616No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT428150.14900474643544034No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC419780.14609181935926463No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT395750.1377288996889537No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG392040.1364377456324887No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC389180.13544240854313833No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG360740.12554472084344445No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG348750.12137196150732178No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG348390.1212466743212497Illumina Single End PCR Primer 1 (100% over 24bp)
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC346030.120425347212555No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC342680.11925948034216208Illumina Single End PCR Primer 1 (100% over 40bp)
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT316220.11005087216586465No Hit
ATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT298720.10396052284291658No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT298710.10395704264330348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)