FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6020076
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT5831259.686339508006212TruSeq Adapter, Index 21 (97% over 40bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2029233.3707713988992833Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1432752.379953342781719Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1043121.7327356000156806Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC800351.3294682658491355Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT766831.273787905667636TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT572880.9516158932212816Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT539150.8955867002343493TruSeq Adapter, Index 21 (100% over 37bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA438020.72759878778939Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCG358700.5958396538515461TruSeq Adapter, Index 21 (100% over 39bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG177550.29492983145063284Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGA167690.2785513006812539TruSeq Adapter, Index 21 (97% over 40bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC167540.27830213439165885No Hit
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC158300.26295349095260595Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT141710.23539569932339724No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC139530.231774482581283No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG136700.22707354525092374Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC120450.20008053054479713Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGG116050.19277165271667668No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC88720.1473735547524649Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTG81930.13609462737679723No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG77830.12928408212786682No Hit
CTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGG75080.12471603348529156No Hit
GTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCG73070.12137720520471836No Hit
CTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGC69870.11606165769335802No Hit
CTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGA68180.11325438416392086No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC66940.11119460950326873No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG66250.11004844457113167No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT65530.10885244638107558TruSeq Adapter, Index 1 (97% over 35bp)
CCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTC63540.10554684027244839No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA60210.10001534864343906No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)