FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49465546
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1140915623.064854070346257TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC26121415.28072812539055TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATG4455440.9007158234945997TruSeq Adapter, Index 8 (97% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1994570.4032240946051622No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAGCTCGTATG652230.1318554130586166TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC622200.1257845208056533TruSeq Adapter, Index 8 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATGTCGTATG575000.11624252565614053TruSeq Adapter, Index 8 (97% over 49bp)
CCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCCGCCCCGAC567420.11471014592662133No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATG517160.10454953837970371TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAACCTCGTATG507780.10265326900465224TruSeq Adapter, Index 8 (97% over 49bp)
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT496290.10033044010067128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)