FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20082471
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT4646982.313948318411614TruSeq Adapter, Index 21 (97% over 40bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1447080.7205686989414799Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1385700.6900047309915199Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1010480.5031651732498456No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT951090.47359211921680355No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT850040.42327460599843514TruSeq Adapter, Index 1 (97% over 35bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT813030.40484559893053No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC765350.38110350065985404No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA720920.3589797291379134No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT718330.35769004720584435No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT708670.35287988216191124No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG639480.3184269505480675No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT616160.30681483369252716No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG610310.30390184554480376No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC584990.2912938353054263No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG517670.25777206400547026Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT493230.24560224685498114TruSeq Adapter, Index 21 (100% over 37bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA465760.23192365122797887No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCG441100.21964428580526768TruSeq Adapter, Index 21 (100% over 39bp)
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT410190.20425275355806563No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA407710.20301784576210766No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC400340.199347978642668No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA352710.175630777706588No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG297310.14804453097430093No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG267130.1330164998121994No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC254640.12679714563013686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC242450.12072717545564987No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC235510.11727142541373518No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC227140.11310361160237702No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC223730.1114056133829348No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT221640.11036490479682504No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA209280.1042102836847119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)