FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30937855
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT3223971.0420793555338597TruSeq Adapter, Index 22 (97% over 40bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1289050.416657845219069No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC481660.1556862943471679No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC336350.10871794440823386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)