FastQCFastQC Report
Wed 27 Sep 2023
EGAF00008245541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128630286
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4822670.37492492242456804Illumina Single End PCR Primer 1 (100% over 50bp)
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC2236050.17383542162069046No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG2208650.17170528564322715No Hit
AGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT2155710.16758961415976328No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG1391630.1081883624203401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)