FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59254648
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT26104724.405514315096429TruSeq Adapter, Index 22 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA2189080.36943599766215807TruSeq Adapter, Index 22 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT635320.10721859321483102TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)