FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80141076
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA42499055.303029622412357TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT11736911.4645311225918654TruSeq Adapter, Index 25 (97% over 44bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2231550.278452712564029No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1797590.22430320251752045No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1627730.20310807905798517No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1202650.15006661502772936No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC890730.11114525090728754No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)