FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73666063
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG36759694.990044058686833TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC9123961.2385567557750439TruSeq Adapter, Index 7 (100% over 50bp)
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC3474480.47165273376968714No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT3359190.4560023792774157No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA3162460.42929673057185097No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2349690.31896505722044627No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1881050.2553482463152673No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1520930.20646277784656417No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1394350.18927983160984183No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1095360.1486926211870451No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG967910.13139157443502852No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT793810.10775789660430203No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)