FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50716460
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC9949461.9617812441956712TruSeq Adapter, Index 1 (100% over 50bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1853810.36552432878793195No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1696430.3344929831459057No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1684860.33221167250237893No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1392670.2745992129576867No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1309930.25828498282411666No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1117840.22040970525151007No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1080620.21307086496178954No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1008510.19885260130537505No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC971150.19148615656534387No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA965350.19034254362390435No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG943790.18609145827607054No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT895340.17653834672214896No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA791980.15615837540711636No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC675410.13317372703063265No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT644910.12715990035582136TruSeq Adapter, Index 1 (100% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA548770.10820352997823587TruSeq Adapter, Index 1 (100% over 37bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC532160.10492845912352716Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA516610.10186239339259877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)