FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48235298
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT10350102.1457522663175004TruSeq Adapter, Index 21 (97% over 40bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2404930.4985830086506359Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1670810.3463874111444279No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1664560.3450916795414014Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1604380.33261533908218005No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1511570.313374243069878No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1080470.22399986001952346No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1033410.21424351934137528No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1028880.21330437307550165Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC885790.18363937546317222No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT852050.17664449797739407TruSeq Adapter, Index 1 (97% over 35bp)
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC804500.1667865719415686No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC793150.1644335233504725No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA784790.16270035275826428No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT777270.16114132849350285No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC752000.15590242647614616Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT689300.1429036470345845No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC656370.13607669636455857No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT642250.13314937952700115No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT641040.13289852588865525TruSeq Adapter, Index 21 (100% over 37bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA613550.12719938000590356No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC606210.12567767281130926No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG574250.11905181968607303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)