FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58072099
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT2448680.4216620446249067TruSeq Adapter, Index 3 (97% over 36bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1397530.24065429424205936No Hit
CTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATG1030040.17737261399833334No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT998610.1719603763590498No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT687960.11846652899527534No Hit
CGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAG680340.11715436702227691No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC664120.11436128733697055No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA614200.10576507661622493No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA581430.10012209133339574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)