FastQCFastQC Report
Tue 7 May 2024
EGAF00008251252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008251252
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences63374
Total Bases15.6 Mbp
Sequences flagged as poor quality0
Sequence length31-4191
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGCCCACAGTGACCATCTCCCCATCCAGGACAGAGGCCCTCAACCAC31344.945245684350049No Hit
CCAGCAACAGTGCCCAGGGCTCTGATGAGTCTCTCATCGCTTGTAAAG24953.9369457506232837No Hit
TCCATCCTAAGGTGACTGTGTATCCTTCAAAGACCCAGCCCCTGCAGCAC20293.201628428061981No Hit
GCTCCCACTCCATGAGGTATTTCTACACCGCCATGTCCCGGCCCGGCCGC19113.015432196168776No Hit
AACACCCAAAGACACACGTGACCCACCATCCCGTCTCTGACCATGAGGCC17322.7329819799917945No Hit
CACGTTTCTTGGAGTACTCTACGTCTGAGTGTCATTTCTTCAATGGGACG16462.597279641493357No Hit
CCTCCAAGAAGGGGCCCCTGCAGCACCACAACCTGCTTGTCTGCCACGTG15902.5089153280525136No Hit
ACCCCCCCAAGACACATATGACCCACCACCCCATCTCTGACCATGAGGCC13562.139678732603276No Hit
CCTCCAAGAAGGGGCCCTTGCAGCACCACAACCTGCTTGTCTGCCACGTG12992.049736484993846No Hit
CTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTTACGGTCCC12912.037113011645154No Hit
AGAATTACCTTTTCCAGGGACGGCAGGAATGCTACGCGTTTAATGGGACA12171.920345883169754No Hit
CACGTTTCTTGGAGCTGCTTAAGTCTGAGTGTCATTTCTTCAATGGGACG12051.9014106731467164No Hit
ACCCCCCAAAGACACATGTGACCCACCACCCCATCTCTGACCATGAGGCC11791.8603843847634676No Hit
GCTCCCACTCCATGAGGTATTTCGACACCGCCGTGTCCCGGCCCGGCCGC11511.816202228043046No Hit
AGAATTACGTGTACCAGGGACGGCAGGAATGCTACGCGTTTAATGGGACA10831.7089027045791647No Hit
ACCCCCCAAAGACACACGTGACCCACCACCCCGTCTCTGACCATGAGGCC10591.6710322845330892No Hit
ATGGCCGTCATGGCGCCCCGAACCCTCGTCCTGCTACTCTCGGGGGCCCT10551.6647205478587432No Hit
GCTCCCACTCCATGAGGTATTTCTACACCTCCGTGTCCCGGCCCGGCCGC10181.6063369836210433No Hit
ATGGCCGTCATGGCGCCCCGAACCCTCCTCCTGCTACTCTCGGGGGCCCT9451.4911477893142298No Hit
GGTCTCACACCCTCCAGTGGATGTATGGCTGCGACCTGGGGCCCGACGGG9291.465900842616846No Hit
GTTCTCACACCATCCAGATAATGTATGGCTGCGACGTGGGGTCGGACGGG9021.423296620065011No Hit
GCTCCCACTCCATGAGGTATTTCTACACCGCCGTGTCCCGGCCCGGCCGC9001.420140751727838No Hit
ACGCCCCCAAAACGCATATGACTCACCACGCTGTCTCTGACCATGAAGCC8941.410673146716319No Hit
ACCCCCCAAAGACACACGTGACCCACCACCCCATCTCTGACCATGAGGCC8501.3412440432985135No Hit
ACGCCCCCAAGACGCATATGACTCACCACGCTGTCTCTGACCATGAGGCC7591.1976520339571433No Hit
GGTCTCACATCATCCAGAGGATGTATGGCTGCGACCTGGGGCCCGACGGG7481.1802947581026921No Hit
GCTCCCACTCCATGAGGTATTTCTTCACATCCGTGTCCCGGCCCGGCCGC6981.1013980496733677No Hit
GGTCTCACACCCTCCAGAGGATGTTTGGCTGCGACCTGGGGCCGGACGGG6961.0982421813361947No Hit
CTGACCACGTCGCCTCTTATGGTGTAAACTTGTACCAGTCTTACGGTCCC6921.0919304446618487No Hit
GGTCTCACACCCTCCAGAGCATGTACGGCTGCGACGTGGGGCCGGACGGG6641.047748287941427No Hit
GCAGCAACAGTGCCCAGGGCTCTGATGAGTCTCTCATCACTTGTAAAG6421.0130337362325244No Hit
GGTCTCACACCCTCCAGAGGATGTACGGCTGCGACGTGGGGCCGGACGGG6180.9751633161864486No Hit
ATGGCCGTCATGGCGCCCCGAACCCTCCTCCTGCTACTCTTGGGGGCCCT6150.9704295136806892No Hit
CGGACCATGTGTCAACTTATGCCATGTTTGTACAGACCCATAGACCAACA6040.9530722378262378No Hit
GGTCTCACACCCTCCAGAGGATGTATGGCTGCGACCTGGGGCCCGACGGG6030.9514943036576514No Hit
AACCCCCAAAGACACACGTGACCCACCACCCCCTCTCTGACCATGAGGCC6030.9514943036576514No Hit
CGGACCATGTGTCAACTTATGCCGCGTTTGTACAGACGCATAGACCAACA5900.930981159466027No Hit
AGGATTTCGTGTACCAGTTTAAGGGCCTGTGCTACTTCACCAACGGGACG5800.9152018177801622No Hit
GCTCCCACTCCATGAGGTATTTCTCCACATCCGTGTCCTGGCCCGGCCGC5760.9088900811058163No Hit
GCTCCCACTCCATGAGGTATTTCTCCACATCCGTGTCCCGGCCCGGCAGT5650.891532805251365No Hit
AGGATTTCGTGTTCCAGTTTAAGGGCATGTGCTACTTCACCAACGGGACG5610.885221068577019No Hit
AGGATTTCGTGTACCAGTTTAAGGCCATGTGCTACTTCACCAACGGGACG5500.8678637927225676No Hit
ATGGCCGTCATGGCGCCCCGAACCCTCGTCCTGCTACTCTCGGGGGCTCT5260.829993372676492No Hit
AGGATTTCGTGTACCAGTTTAAGGGCATGTGCTACTTCACCAACGGGACA5200.820525767664973No Hit
CGGACCATGTGTCAACTTATGCCGCGTTTGTACAGACCCATAGACCAACA5120.8079022943162811No Hit
AGGATTTCGTGTACCAGTTTAAGGGCATGTGCTACTTCACCAACGGGACG5020.7921229526304162No Hit
GTTCTCACACCATCCAGAGGATGTATGGCTGCGACGTGGGGCCGGACGGG4850.765298071764446No Hit
CACGTTTCTTGGAGCTGCGTAAGTCTGAGTGTCATTTCTTCAATGGGACG4850.765298071764446No Hit
AGAATTACGTGTACCAGTTACGGCAGGAATGCTACGCGTTTAATGGGACA4690.7400511250670622No Hit
GCTCTCACTCCATGAGGTATTTCTTCACATCCGTGTCCCGGCCCGGCCGC4610.7274276517183703No Hit
CACGTTTCTTGGAGCAGGCTAAGTGTGAGTGTCATTTCCTCAATGGGACG4550.7179600467068513No Hit
GTTCTCACACCATCCAGATGATGTATGGCTGCGACGTGGGGTCGGACGGG4420.697446902515227No Hit
TCCAACCTAAGGTGACTGTATATCCTTCAAAGACCCAGCCCCTGCAGCAC4210.6643102849749108No Hit
GCACGTTTCTTGGAGCAGGCTAAGTGTGAGTGTCATTTCCTCAATGGGAC4210.6643102849749108No Hit
GCTCCCACTCCATGAGGTATTTCTCCACATCCGTGTCCCGGCCCGGCCGC4050.639063338277527No Hit
GCTCCCACTCCATGAGGTATTTCCACACCGCCATGTCCCGGCCCGGCCGC3920.6185501940859027No Hit
GCTCCCACTCCATGAGGTATTTCACCACATCCGTGTCCCGGCCCGGCCGC3750.5917253132199324No Hit
GTTCTCACACCCTCCAGATGATGTTTGGCTGCGACGTGGGGTCGGACGGG3710.5854135765455866No Hit
CACGTTTCTTGCAGCAGGATAAGTATGAGTGTCATTTCTTCAACGGGACG3650.5759459715340676No Hit
CACGTTTCCTGTGGCAGCCTAAGAGGGAGTGTCATTTCTTCAATGGGACG3610.5696342348597216No Hit
CTGACCATGTTGCCTCTTATGGTGTAAACTTGTACCAGTCTTACGGTCCC3410.5380755514879919No Hit
AGAATTACGTGCACCAGTTACGGCAGGAATGCTACGCGTTTAATGGGACA3350.528607946476473No Hit
GCCGCGTTTGTACAGACCCATAGACCAACAGGGGAGTTTATGTTTGAATT3340.5270300123078865No Hit
GCCGCGTTTGTACAGACGCATAGACCAACAGGGGAGTTTATGTTTGAATT3270.5159844731277811No Hit
GTTCTCACACCGTCCAGAGGATGTATGGCTGCGACGTGGGGTCGGACTGG3180.5017830656105027No Hit
TCCATCCTGAGGTGACTGTGTATCCTGCCAAGACTCAGCCCCTGCAGCAC3170.5002051314419163No Hit
GTTCTCACACCATCCAGAGGATGTATGGCTGCGACCTGGGGCCCGACGGG3100.4891595922618108No Hit
GGTCTCACACTTGGCAGAGGATGTATGGCTGCGACCTGGGGCCCGACGGG3070.4844257897560514No Hit
AACGCCCAAAGACACACGTGACCCACCATCCCGTCTCTGACCATGAGGCC3000.473380250575946No Hit
GCTCCCACTCCATGAGGTATTTCTACACCGCTGTGTCCCGGCCCGGCCGC2990.4718023164073595No Hit
CACGTTTCTTGGAGTACTCTACGGGTGAGTGTTATTTCTTCAATGGGACG2940.4639126455644271No Hit
CTGACCATGTTGCCTCTTACGGTGTAAACTTGTACCAGTCTTATGGTCCC2930.4623347113958406No Hit
GCCATGTTTGTACAGACCCATAGACCAACAGGAGAGTTTATGTTTGAATT2920.4607567772272541No Hit
AACACCCAAAGACACACGTGACCCACCATCTCGTCTCTGACCATGAGGCC2850.4497112380471487No Hit
GGTCTCACACCCTCCAGAGGATGTACGGCTGCGACCTGGGGCCCGACGGG2780.4386656988670433No Hit
GCTCCCACTCCATGAGGTATTTCTACACCGCTGTGTCCCGGCCCAGCCGC2770.4370877646984568No Hit
GCTCCCACTCCATGAGGTATTTCTACACCTCCATGTCCCGGCCCGGCCGC2710.4276201596869379No Hit
GTTCTCACACCATCCAGATGATGTATGGCTGCGACGTGGGGCCGGACGGG2700.4260422255183514No Hit
CTGACCACGTTGCCTCTTACGGTGTAAACTTGTACCAGTCTTACGGTCCC2490.3929056079780352No Hit
GTTCTCACACCCTCCAGAGGATGTATGGCTGCGACGTGGGGTCGGACTGG2440.38501593713510274No Hit
GCCATGTTTGTACAGACCCATAGACCAACAGGGGAGTTTATGTTTGAATT2400.37870420046075676No Hit
AGGATTTCGTGTTCCAGTTTAAGGGCATGTGCTACTTCACCAACGGGACC2390.3771262662921703No Hit
GTTCTCACACCATCCAGATAATGTATGGCTGCGACGTGGGGCCGGACGGG2330.3676586612806514No Hit
GCTCCCACTCCATGAGGTATTTCCACACCTCCGTGTCCCGGCCCGGCCGC1910.3013854262000189No Hit
CACGTTTCCTGTGGCAGGGTAAGTATAAGTGTCATTTCTTCAACGGGACG1870.29507368952567303No Hit
TTGAGCCTAAGGTGACTGTGTATCCTTCAAAGACCCAGCCCCTGCAGCAC1860.2934957553570865No Hit
GGTCTCACACCCTCCAGAGGATGTCTGGCTGCGACCTGGGGCCCGACGGG1840.29033988701991353No Hit
CTGACCATGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTTACGGTCCC1830.28876195285132705No Hit
ACCCCCCAAAGACACATGTGACCCACCACCCCATCTCTGACCATGAGGTC1790.28245021617698113No Hit
ACCCCCCCAAGACGCATATGACTCACCACGCTGTCTCTGACCATGAGGCC1530.24142392779373245No Hit
CACGTTTCTTGTGGCAGCTTAAGTTTGAATGTCATTTCTTCAATGGGACG1510.23826805945655946No Hit
GGTCTCACACTTGGCAGACGATGTATGGCTGCGACGTGGGGCCGGACGGG1500.236690125287973No Hit
CACGTTTCTTGGAGCAGGTTAAACATGAGTGTCATTTCTTCAACGGGACG1490.2351121911193865No Hit
GGTCTCACATCATCCAGGTGATGTATGGCTGCGACGTGGGGCCGGACGGG1440.22722252027645407No Hit
TCTATCCTGAGGTGACTGTGTATCCTGCAAAGACCCAGCCCCTGCAGCAC1430.22564458610786758No Hit
GGTCTCACATCATCCAGAGGATGTACGGCTGCGACGTGGGGCCGGACGGG1370.21617698109634864No Hit
GGTCTCACATCATCCAGAGGATGTACGGCTGCGACGTGGGGCCCGACGGG1210.19093003439896486No Hit
GCTCCCACTCCATGAGGTATTTCGACACCGCCATGTCCCGGCCCGGCCGC1110.17515069271310002No Hit
TTCAACCTAAGGTGACTGTGTATCCTTCAAAGACCCAGCCCCTGCAGCAC1040.1641051535329946No Hit
GCTCTCACTCCATGAGGTATTTCTACACCTCCGTGTCCCGGCCCGGCCGC1010.15937135102723513No Hit
CACGTTTCTTGGAGGAGGTTAAGTTTGAGTGTCATTTCTTCAACGGGACG1000.15779341685864867No Hit
TTTCTTGGAGTACTCTACGTCTGAGTGTCATTTCTTCAATGGGACGGAGC990.15621548269006216No Hit
CACGTTTCTTGAAGCAGGATAAGTTTGAGTGTCATTTCTTCAACGGGACG980.15463754852147568No Hit
ATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGCTCTCGGGAGCCCT870.13728027266702433No Hit
TGTACCAGTTTAAGGCCATGTGCTACTTCACCAACGGGACGGAGCGCGTG860.13570233849843785No Hit
GGTCTCACATCCTCCAGAGGATGTATGGCTGCGACGTGGGGCCCGACGGG810.1278126676555054No Hit
GGTCTCACACCCTCCAGTGGATGTATGGCTGCGACGTGGGGCCGGACGGG790.12465679931833243No Hit
ATGCGGGTCACGGCGCCCCGAACCGTCCTCCTGCTGCTCTGGGGGGCAGT680.1072995234638811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)