FastQCFastQC Report
Wed 8 May 2024
EGAF00008251253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008251253
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences11064
Total Bases31.2 Mbp
Sequences flagged as poor quality0
Sequence length339-5705
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCCTCGTCCTGC7076.390093998553868No Hit
TCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCCTCCTCCTGC7016.335864063629789No Hit
CACCCACCCGGACTCAGAATCTCCTCAGACGCCGAGATGCGGGTCACGGC6555.920101229211858No Hit
TAGGGAGGGGGGTCCCAAAAGCCTGGGGATCAGACGTGGTTTTCCCGCCT5945.368763557483731No Hit
GCCATCTGTGAGCATTTAGAATCCTCTCTATCCTGAGCAAGGAGTTCTGA5014.528199566160521No Hit
AATACGCGTGTCAGAACGAGGCCAGGCTTAATTCCTGGACCTATCTCGTC3763.398409255242227No Hit
ACAATCTCAAATTCTATTTCATTATTTTTCTTCCACGCTCCTAGTCCAGC3533.190527838033261No Hit
ACGGATTCTCACTCTGTCGCCCAGGCCGGAGTGCAGTGGCGCGATCTCGG2912.6301518438177873No Hit
TCGCAGAGGATTTCGTGTACCAGTTTAAGGGCATGTGCTACTTCACCAAC2792.5216919739696313No Hit
GTCCGCCCCCTCCCCGCAGAGAATTACCTTTTCCAGGGACGGCAGGAATG2762.4945770065075923No Hit
CCGCAGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCGGATTCT2432.1963123644251628No Hit
CCGCAGAGGATTTCGTGTACCAGTTTAAGGCCATGTGCTACTTCACCAAC2372.1420824295010847No Hit
CGCAGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCGGATTCTC2312.0878524945770067No Hit
CACCCACCCGGACTCAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGC2292.0697758496023138No Hit
GAAAGAATAAAGCAAAAAAAGCAGAGATTTATTGAAAATGAAAGTACACT2282.0607375271149677No Hit
AATACGCGTATCAGAACGAGGACAAGATTAATTCCTGGACCTGTCGCATC2232.0155459146782357No Hit
GTCCGCCCCCTCCCCGCAGAGAATTACGTGTACCAGGGACGGCAGGAATG1871.6901663051337672No Hit
ACAATCTCAAATGCTATTTCATTATTTTTCTTCCCCACTCGTAGTCCAGC1811.635936370209689No Hit
GCCATCTGTGAGCATTTGGAATCCTCTCTATCCTGAGCAAGGAGTTCTGA1691.527476500361533No Hit
GAAAGAATAAAGCAACAAAAGCAAAGATTTATTGAAAATGAAAGTACACT1541.3919016630513377No Hit
CGCAGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCAGATTCTC1461.319595083152567No Hit
CGCAGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCACATTCTC1461.319595083152567No Hit
CTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTTACGGTCCC1461.319595083152567No Hit
TTTCTTCCTTTTTATATTCATAAGTCTTTATTCATTCTCTAGCTTTGACC1351.2201735357917571No Hit
CACCCACCCGGACTCAAAATCTCCTCAGACGCCGAGATGCGGGTCACGGC1341.2111352133044107No Hit
ACAATCTCAAATGCTATTTCATTATTTTCCTTCCCCACTCGTAGTCCAGC1341.2111352133044107No Hit
CCGCAGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCACATTCT1291.165943600867679No Hit
CCCACCCGGACTCAGAATCTCCTCAGACGCCGAGATGCGGGTCACGGCGC1271.1478669558929864No Hit
ACCCACCCGGACTCAGAATCTCCTCAGACGCCGAGATGCGGGTCACGGCG1181.0665220535068691No Hit
CTTCTTCCTTTTTATATTCATAAGTCTTTATTCATTCTCTAGCTTTGACC1050.9490238611713666No Hit
CCGCAGAGGATTTCGTGTTCCAGTTTAAGGGCATGTGCTACTTCACCAAC980.8857556037599421No Hit
TTCTTCCTTTTTATATTCATAAGTCTTTATTCATTCTCTAGCTTTGACCA960.8676789587852495No Hit
CCGCAGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCAGATTCT910.8224873463485178No Hit
GTCCGCCCCCTCCCCGCAGAGAATTACGTGTACCAGTTACGGCAGGAATG900.8134490238611713No Hit
GTCCGCCCCCTCCCCGCAGAGAATTACGTGCACCAGTTACGGCAGGAATG860.777295733911786No Hit
GATAAGGCATGAGACAGAAGTAATAGCAAAGACATTGGATCCAAATTTCT820.7411424439624006No Hit
GCCATCTGTGAGCATTCAGAATCCTCTCAATCCTGAGCAAGGAGTTCTGA820.7411424439624006No Hit
GCCATCTGTGAGCATTTAGAATCCTCTCTATCCTGAGCAAGGAGTTCTGC820.7411424439624006No Hit
GAAAGAATTAAGCAACAAAAGCACAGATTTATTAAAAAAGAAAGTACACT810.7321041214750542No Hit
CTGACCACGTCGCCTCTTATGGTGTAAACTTGTACCAGTCTTACGGTCCC810.7321041214750542No Hit
CAGGGAGGGGGTCCCAAAGCCTGGGGATCAGAGGTAGTTTTCCCACCTGG720.6507592190889371No Hit
CCGCAGAGGATTTCGTGTACCAGTTTAAGGGCATGTGCTACTTCACCAAC650.5874909616775127No Hit
CCCACCCGGACTCAGAATCTCCTCAGACGCCGAGATGCGGGTCACGGCAC640.5784526391901663No Hit
ACCGGATCGTTCTTGTCCCCCCAGCACGTTTCTTGGAGCAGGTTAAACAT600.5422993492407809No Hit
CCCACCCGGACTCAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGC560.5061460592913956No Hit
GCCATCTGTGAGCATTTAGAATCCTCTCAGTCCGGAGCAAGCAGTTCTGA540.4880694143167028No Hit
CTCTGTCGCCCAGGCTGGAGTGCAGTGGCGGGATCTCGGCTCACTGCAAG530.47903109182935644No Hit
CAGGGAGGGGGTCCCAAAAGCCTGGGGATGAGAAGGGGTTTTCCCGCATG520.4699927693420101No Hit
TCCCCAGACGCCGAGAATGGCCGTCATGGCGCCCCGAACCCTCGTCCTGC500.45191612436731743No Hit
ACCCACCCGGACTCAGAATCTCCTCAGACGCCGAGATGCGGGTCACGGCA470.42480115690527837No Hit
CAGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCGGATTCTCCC460.41576283441793205No Hit
CGCAGTCCCGGTTCTGAAGTCACCCACCCGGACTCAGATTCTCCCCAGAC460.41576283441793205No Hit
CGCAGTCCCGGTTCTGAAGTCCCCAGTCACCCACCCGGACTCAGATTCTC440.39768618944323936No Hit
CTGACCATGTTGCCTCTTACGGTGTAAACTTGTACCAGTCTTATGGTCCC440.39768618944323936No Hit
CCGCAGTCCCGGTTCTGAAGTCCCCAGTCACCCACCCGGACTCAGATTCT430.388647866955893No Hit
ATACGCGTGTCAGAACGAGGCCAGGCTTAATTCCTGGACCTATCTCGTCA420.3796095444685466No Hit
CTGACCATGTTGCCTCTTATGGTGTAAACTTGTACCAGTCTTACGGTCCC390.35249457700650755No Hit
GCAGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCGGATTCTCC380.34345625451916123No Hit
ACCCACCCGGACTCAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCG380.34345625451916123No Hit
CAGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCACATTCTCCC350.31634128705712217No Hit
GATAAGGCATGAGACAGAAGTAACAGCAAAGACATTGGATCCAAATTTCT340.30730296456977585No Hit
CTGACCATGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTTACGGTCCC340.30730296456977585No Hit
GCCATCTGTGAGCATTTAGAATCCTCTCAATCTTGAGTAAGGAGTTCTGA340.30730296456977585No Hit
ACGCAGTCCCGGTTCTGAAGTCACCCACCCGGACTCAGATTCTCCCCAGA340.30730296456977585No Hit
CCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCCTCGTCCTGCT280.2530730296456978No Hit
CTGACCACGTTGCCTCTTACGGTGTAAACTTGTACCAGTCTTACGGTCCC280.2530730296456978No Hit
CACCCACCCGGACTCAGAGTCTCCTCAGACGCCAAGATGCTGGTCATGGC280.2530730296456978No Hit
CCCACCCGGACTCAAAATCTCCTCAGACGCCGAGATGCGGGTCACGGCGC270.2440347071583514No Hit
ATACGCGTATCAGAACGAGGACAAGATTAATTCCTGGACCTGTCGCATCA270.2440347071583514No Hit
ACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTTACGGTCCCTCT270.2440347071583514No Hit
TAGGGACGGGGGTCCCAAAAGCCTGGGGATCAGACGTGGTTTTCCCGCCT260.23499638467100506No Hit
CAGGGAGGGGGTCCCAAAAGCCTTGGGATCAGAAGGGGTTTTCCCGCCTG240.21691973969631237No Hit
CCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCCTCCTCCTGCT230.20788141720896602No Hit
ACCCACCCGGACTCAAAATCTCCTCAGACGCCGAGATGCGGGTCACGGCG210.1898047722342733No Hit
CCACCCGGACTCAGAATCTCCTCAGACGCCGAGATGCGGGTCACGGCGCC200.18076644974692696No Hit
GCAGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCACATTCTCC200.18076644974692696No Hit
GCAGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCAGATTCTCC200.18076644974692696No Hit
CAGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCAGATTCTCCC190.17172812725958062No Hit
TGATTCCCCGCAGAGGATTTCGTGTACCAGTTTAAGGCCATGTGCTACTT190.17172812725958062No Hit
AGGGAGGGGGGTCCCAAAAGCCTGGGGATCAGACGTGGTTTTCCCGCCTG190.17172812725958062No Hit
GCCATCTGTGAGCATTTAGAATCCTCTCAATCCCCAGCAAGCAGTTCTGA180.16268980477223427No Hit
CCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCCTCGTCCTGCTA170.15365148228488792No Hit
ACCACGTCGCCTCTTATGGTGTAAACTTGTACCAGTCTTACGGTCCCTCT170.15365148228488792No Hit
AGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCGGATTCTCCCC170.15365148228488792No Hit
GCAGTCCCGGTTCTGAAGTCACCCACCCGGACTCAGATTCTCCCCAGACG160.14461315979754158No Hit
CAGTCCCGGTTCTGAAGTCACCCACCCGGACTCAGATTCTCCCCAGACGC150.13557483731019523No Hit
AGTCCCGGTTCTAAAGTCCCCAGTCACCCACCCGGACTCACATTCTCCCC150.13557483731019523No Hit
TCCCCAGACGCCGAGGATGGCCGTCATGCCGCCCCGAACCCTCCTCCTGC140.1265365148228489No Hit
CCACCCGGACTCAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCC130.11749819233550253No Hit
CCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCCTCCTCCTGCTA120.10845986984815618No Hit
GCCATCGGTGAGCATTTAGAATCCTCTCTATCCTGAGCAAGGAGTTCTGA120.10845986984815618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)