FastQCFastQC Report
Thu 16 May 2024
EGAF00008256344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008256344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93151984
Total Bases8.4 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTC55770225.9870136528707745Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGA29490983.1658992899174323Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAG14747721.583189038678983Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA12616071.3543533329359898Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA10963001.1768938812940366Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT10730711.1519572143519778Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCG9545491.0247221358162377Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT8981270.9641523040453974Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGA7128980.7653062977166434Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA6342530.6808797545310468Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACGACCCATTCGAACG5125950.5502781347094013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAA5008030.5376192524251551Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAAC3788590.4067106074734812Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3182020.34159444204645173Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3178120.34117577141459493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGGGGCGGGCGCCGG2723020.29232012922022144Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGC2495890.26793739572954234Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCT2084440.2237676440686438Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA1876500.2014449847895886Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTC1817570.19511876419078739Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAA1716890.18431062080223648Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACCGCGGTTCTATTTTG1692990.1817449212890624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA1688660.1812800895362572Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1663610.1785909358624074Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGGCGGCGACGACCCATT1555370.16697121555671857Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATT1542510.1655906759860316Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA1526120.16383118581779216Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG1455560.15625646792450498No Hit
CAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAA1424470.15291891152849735Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCG1364990.14653364763545992Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGCGGCGACGACCCATTCG1308970.14051981973889038Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCT1298110.13935398305633512No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAA1200120.12883461505232138Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCC1147190.12315250311791534No Hit
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC1137450.12210690005271387Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAA1102700.11837643737142517Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC1089940.1170066329451448No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAA1066260.1144645507496652Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTG1036620.11128265394755306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCCTGGATACCGCAGC1022230.10973786666744532Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGCCGGGCGCGACCCGC1017530.10923331488033577Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGC1008880.10830472488916607No Hit
GTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACG1005400.10793114186381687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC989410.10621459227320375Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACC949300.10190872585172206No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)