FastQCFastQC Report
Wed 8 May 2024
EGAF00008266817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008266817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862350
Total Bases143.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1267394.427795342987405TruSeq Adapter, Index 27 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG859393.0023931385050746TruSeq Adapter, Index 27 (97% over 38bp)
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC35470.12391915733575558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)