FastQCFastQC Report
Wed 8 May 2024
EGAF00008268938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008268938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287803
Total Bases114.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC31010.13554488738759413No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA27380.1196781366227774No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG26560.11609391193210254TruSeq Adapter, Index 7 (97% over 34bp)
CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG25000.10927514300837966No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA23770.10389880597236738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)